Biolix Technologies Incepted in the year 2010, we at Biolix Technologies are committed to bring in innovative & cost effective solutions to the Biotech industry. Backed by years of knowledge about the Biotech industry and the skills to reach out to the industry majors we have achieved an enviable position in a short period of time. We provide products and services to the Academic & industrial research institutions in the Biotech industry, and are importers and marketers of biological, biochemical and lab equipment for life science researchers,pharmaceutical and biotech industries, food industries. Our services are related to Biotechnology, Life Sciences, Health Care, Automobile, Quality Control etc. We take immense care and consideration to deliver products and services that are as per the customer’s requirements.We are a team of people with wide experience in marketing and have worked with world class companies. We strive to deliver products and services which are of International standards. We identify the customer needs and deliver products and services as per their requirement which makes us stand a class apart. Our success is measured through satisfied stakeholders enjoying the benefit of a stable and viable organization, which maintains effectiveness and strives for continual improvement. Guided by commitment to our customers, ourselves and our stakeholders, we shall always maintain high standards when conducting business.

Steripak™-GP Pressure Driven Filter Units Steripak-GP filter units are designed for larger scale pressure-driven filtration of tissue culture media with or without serum. They are single use and are not autoclavable. The filter units come in two sizes, with the smaller unit able to filter up to 10 L and the larger up to 20 L. Both sizes utilize the low protein binding, fast flow 0.22 µm Millipore Express membrane. Features & Benefits Millipore Express® PLUS membrane for fastest flow rates and low protein binding. Larger scale, pressure-driven filtration device for faster flow rates speeds time-consuming, large-volume filtrations. 10 L and 20 L capacity sizes for maximum laboratory-scale filtration options. Manually operated top air vent to prevent air lock stop of flow Filling bell for cleaner filling environment around the opening of the receptacle bottle Applications Sterilization of Aqueous Solutions, Media Preparation, Buffer Preparation

Scepter™ 2.0 Handheld Automated Cell Counter The Scepter™ 2.0 Handheld Automated Cell Counter provides accurate cell counts in 30 seconds. The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. Prepare a dilution of the cell culture of interest and use the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor. The Scepter cell counter’s screen displays: • Cell concentration • Average cell size • Average cell volume • Histogram of size or volume distribution This system is intended for research use only and has been tested with cell types representative of those in use today.

PureProteome™ magnetic beads from Merck Millipore Complete Recovery with Reproducible Results Merck’s new PureProteome™ magnetic beads ensure the rapid and reproducible isolation of proteins. Unlike conventional methods that require centrifugation to pellet the beads followed by careful aspiration to avoid sample loss, PureProteome™ magnetic beads are isolated using a magnetic rack. This allows for the total removal of buffers for complete recovery of beads with no sample dilution. You eliminate variability while maximizing recovery. Advantages of PureProteome™ Beads •High capacity: Advanced chemistry combined with high surface area provides more binding sites for proteins. •Consistent results with no sample loss: Particles visible as they adhere to side of tube for quick and easy aspiration and complete buffer removal •Fast processing time: Beads are immobilized in seconds. Increased kinetics of bead-protein binding enables shorter incubations •Economical: Significantly more affordable then competitive magnetic beads Beads available include: • Protein A • Protein G • Protein A/G Mix • Kappa Ig-Binder • Lambda Ig-Binder • Streptavidin • Nickel • Albumin • Albumin/IgG Depletion Kit • Human Albumin/Immunoglobulin Depletion • NHS FlexiBind • Carboxy FlexiBind

Measuring Protein Biomarkers?? Biolix provide multiple options for protein/biomarker detection and quantification. We help you reduce error bars with single and multiplex protein analysis solutions. The various options that we can provide include: MILLIPLEX® map multiplex detection platforms This system offers the broadest selection of analytes across a wide range of research areas and species. SMC™ assays for use with SMCxPRO™ or Erenna® instruments This system quantify concentrations down to the femtogram/mL level. ELISA Kits Quantify your critical targets with classic assay kits. We provide a combined offering of >1800 ELISAs, EIAs and RIAs against numerous soluble circulating and intracellular biomarkers. GyroMark™ HT Kits High-throughput, nanoliter-scale assays requiring as little as 1 μL of sample. Our custom kits for the Gyrolab® platform are fully validated for reliable use in translational research, with accuracy and precision that meets or exceeds ELISA performance. Custom Premix Kit Option for MILLIPLEX® map Panels We offer bead mixing for selected MILLIPLEX® map kits. • Available for any number of kits • Available for any number of analytes within a qualifying kit • Premixed vials are quality assurance tested • Allow up to 10 additional business days

Guava® easyCyte Flow Cytometers Flexible, Intutive & Affordable Guave easyCyte Flow Cytometers are microcapillary flow cytometry systems and are far easier to maintain. It utilizes small sample volumes, generate minimal waste, and have lower operating costs. As a result, Guava® easyCyte flow cytometers are uniquely amenable to on-demand use in the laboratory environment and have helped many scientists achieve insightful cellular analysis since 1998. Advantages: • Upto 14 available detection channels • Intutive sofmware interface • Powerful analysis tools • Ease of single sample loading • High throughput efficiency of walkaway acquisition from multiwell paltes

CRISPR/CAS9 Technology Opens a New Era in Gene Editing CRISPR/Cas9 is a revolutionary gene editing technique that can modify any region of the genome of any species with high precision and accuracy and it does so without harming other genes. Have you ever wondered how the bacteria defend themselves against viral attacks? Bacteria has got a small RNA sequence in their body which is complimentary to the targeted sequence of DNA from Virus. When virus attack bacteria, the viral DNA is released to bacteria. The short RNA sequence called ‘Guide RNA’ present in bacteria, identifies the viral DNA sequence and binds to it. Bacteria also has a Cas 9 nuclease present inside which then binds to the RNA DNA complex and cleaves the targeted DNA sequence. Scientists have utilised this wonderful mechanism present in Bacteria to perform the following: • Delete a Gene: Undesirable genes can be deleted from the genome, allowing researchers to study the functions specific to the genes and learn about what happens to the cell when these genes are not in the genome • Add a New Gene: Desirable genes can be added into the genome, allowing researchers to study their functions within the cells. These genes can also add new functions to the cell. • Activate Dead Genes: Genes that are essential for various functions, but no longer function, can be reactivated using CRISPR-Cas9 system. • Control Gene Activity Level: Genes are more active than normal can be controlled to produce just the right amount of proteins, which will help maintain balance within the cell under desired conditions. Why CRISPR-Cas 9? • Human Health: The CRISPR-Cas9 system can revolutionize gene therapy and make it possible to treat a large number of diseases that would be impossible to treat without this technique. • New Materials: Manipulating biological circuits using CRISPR-Cas9 will facilitate the generation of synthetic materials that could be useful in various applications, such as oral drug delivery and the production of biosensors. • Drug Development: Engineering cells to optimize a high yield generation of drug precursors in bacterial factories could significantly reduce the cost and accessibility of useful therapeutics • Research Applications: The CRISPR-Cas9 system could allow the creation of new animal and cellular models, which will help us learn more about diseases and test new drugs and vaccines on these models. • Agriculture: CRISPR gene editing tools can edit crops without harming other genes, which will help confer resistance to infections and harsh environments, improving global food security. • Bioenergy: Sustainable and cost-effective biofuels are attractive sources for renewable energy, which could be achieved by creating efficient metabolic pathways for ethanol production in algae or corn.

Steripak-GP filter units-For larger scale pressure driven Filtration[10-20 L] Steripak-GP filter units are designed for larger scale pressure-driven filtration of tissue culture media with or without serum. They are single use and are not autoclavable. The filter units come in two sizes, with the smaller unit able to filter up to 10 L and the larger up to 20 L. Both sizes utilize the low protein binding, fast flow 0.22 µm Millipore Express membrane. Each unit has an integral filling bell for bottle-top use and a manually operated upstream air vent to prevent membrane air locking. They are supplied sterile and ready to connect to a pump or pressure vessel (not included). Features & Benefits Millipore Express® PLUS membrane for fastest flow rates and low protein binding Larger scale, pressure-driven filtration device for faster flow rates speeds time-consuming, large-volume filtrations 10 L and 20 L capacity sizes for maximum laboratory-scale filtration options Manually operated top air vent to prevent air lock stop of flow Filling bell for cleaner filling environment around the opening of the receptacle bottle Applications Sterilization of Aqueous Solutions, Media Preparation, Buffer Preparation

pET Expression Vectors Now efficiently clone and express your target protein !!! Merck Millipore provides vast portfolio of expression vectors which enable you to choose the perfect combination of promoters, epitope tags, antibiotic resistance, and host compatibility. . The pET System continuously offer new technologies and options for expression. Wide variety of pET vector types and host strains enable high level expression, purification and detection of target proteins. A few of popular Novagen pET vectors includes the following: high yield bioproduction of peptides and small proteins [pET-31b(+)] production of soluble, active target proteins in E. coli [pET-32a(+), pET-32b(+), pET-32c(+)] production of target proteins suitable for site-specific 32P-labeling [pET-33b(+)] Dsb tags for export and periplasmic folding of target proteins [pET-39b(+), pET-40b(+)] with popular GST fusion tags for enhanced production and solubility [pET-41a(+), pET-41b(+), pET-41c(+) , pET-42a(+), pET-42b(+), pET-42c(+)] with amino-terminal His•Tag™ sequence and minimal extraneous sequences [pET-45b(+)]

Immobilong PVDF Membranes Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. Features & Benefits - Won’t crack, curl or fracture when cut - Low background - Superior staining capabilities - Can be reprobed multiple times Immobilon-P Transfer Membrane - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity Immobilon-E Membrane - The only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa Immobilon-PSQ Membrane - 0.2 µm pore size and a large internal structure - Higher protein adsorption and sequencing yields than other membranes - Recommended for blotting proteins less than 20 kDa - Prevents blow-through of low molecular weight proteins Immobilon-FL Membrane - The first transfer membrane optimized for fluorescence applications - Extremely low background improves sensitivity of all fluorescence detection protocols - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths - Ideal for multiplexing and chemifluorescence applications

Automation Compatible Packaging Automation compatible filter units from Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications. The filters are designed with a pressure-resistant, domed housing and an optimized Luer-Lok® connection to ensure reliable delivery by automated systems. Features & Benefits • Domed housing ensures reliable delivery of filters • Available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples. • Pressure-resistant housing resists bursting • Luer-Lok® connection optimized for precise alignment and fit • Available in either bulk or delivery tubes for use with automated filter-changing systems Applications Drug Dissolution Testing Automated Sample Preparation Clarification of Solutions Containing High Particle Levels