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Immobilong PVDF Membranes Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. Features & Benefits - Won’t crack, curl or fracture when cut - Low background - Superior staining capabilities - Can be reprobed multiple times Immobilon-P Transfer Membrane - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity Immobilon-E Membrane - The only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa Immobilon-PSQ Membrane - 0.2 µm pore size and a large internal structure - Higher protein adsorption and sequencing yields than other membranes - Recommended for blotting proteins less than 20 kDa - Prevents blow-through of low molecular weight proteins Immobilon-FL Membrane - The first transfer membrane optimized for fluorescence applications - Extremely low background improves sensitivity of all fluorescence detection protocols - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths - Ideal for multiplexing and chemifluorescence applications